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小鼠膠原酶I酶免試劑盒,(Collagenase I)ELISA檢測(cè)試劑盒 ,英文名:Collagenase I ELISA Kit ,英文縮寫:Collagenase I ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:小鼠膠原酶IELISA檢測(cè)試劑盒,Collagenase I 檢測(cè)試劑盒, Collagenase I 檢測(cè)試劑盒
ELISA實(shí)驗(yàn)操作較繁雜,操作不當(dāng)將引起較大的誤差。小鼠膠原酶I酶免試劑盒,(Collagenase I)ELISA檢測(cè)試劑盒 在操作中應(yīng)注意以下5個(gè)方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機(jī)體內(nèi)的含量發(fā)生大幅波動(dòng),因此有必要對(duì)標(biāo)本進(jìn)行預(yù)處理,例如對(duì)血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測(cè)定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時(shí)必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時(shí)則不需更換吸嘴。
3.在成批手工檢測(cè)中,還應(yīng)注意操作時(shí)差的影響。加樣及混勻時(shí)間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時(shí)間不*,對(duì)競(jìng)爭(zhēng)法及定量檢測(cè)影響很大,不注意可能會(huì)導(dǎo)致錯(cuò)誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實(shí)驗(yàn)成敗的一個(gè)關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C(jī)洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測(cè)讀儀(酶標(biāo)儀),比色法判讀可選擇單波長(zhǎng)或雙波長(zhǎng),根據(jù)反應(yīng)液顏色的不同選用不同波長(zhǎng)的濾光片,以空白孔校零測(cè)定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
血管緊張素1-7(Ang1-7)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Angiotensin 1-7 (Ang1-7)96TY17196Canis familiaris; Canine (Dog)
組織蛋白酶D(CTSD)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Cathepsin D (CTSD)96TY17197Mus musculus (Mouse)
組織轉(zhuǎn)谷氨酰胺酶(TGM2)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Transglutaminase 2, Tissue (TGM2)96TY17198Mus musculus (Mouse)
β-1,4-半乳糖轉(zhuǎn)移酶1(β4GALT1)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Beta-1,4-Galactosyltransferase 1 (b4GALT1)96TY17199Mus musculus (Mouse)
凝血酶原片段F1+2(F1+2)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Prothrombin Fragment 1+2 (F1+2)96TY17200Rattus norvegicus (Rat)
維生素B1(VB1)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Vitamin B1 (VB1)96TY17201General
低氧誘導(dǎo)因子1α(HIF1α)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Hypoxia Inducible Factor 1 Alpha (HIF1a)96TY17202Homo sapiens (Human)
低氧誘導(dǎo)因子1α(HIF1α)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Hypoxia Inducible Factor 1 Alpha (HIF1a)96TY17203Mus musculus (Mouse)
低氧誘導(dǎo)因子1α(HIF1α)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Hypoxia Inducible Factor 1 Alpha (HIF1a)96TY17204Sus scrofa; Porcine (Pig)
低氧誘導(dǎo)因子1α(HIF1α)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Hypoxia Inducible Factor 1 Alpha (HIF1a)96TY17205Rattus norvegicus (Rat)
上皮中性粒細(xì)胞激活肽78(ENA78)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Epithelial Neutrophil Activating Peptide 78 (ENA78)96TY17206Homo sapiens (Human)
上皮中性粒細(xì)胞激活肽78(ENA78)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Epithelial Neutrophil Activating Peptide 78 (ENA78)96TY17207Mus musculus (Mouse)
凋亡蛋白酶激活因子1(APAF1)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Apoptotic Peptidase Activating Factor 1 (APAF1)96TY17208Homo sapiens (Human)
趨化因子C-C-基元配體14(CCL14)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Chemokine C-C-Motif Ligand 14 (CCL14)96TY17209Homo sapiens (Human)
白介素31(IL31)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Interleukin 31 (IL31)96TY17210Mus musculus (Mouse)
白介素19(IL19)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Interleukin 19 (IL19)96TY17211Homo sapiens (Human)
白介素19(IL19)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Interleukin 19 (IL19)96TY17212Mus musculus (Mouse)
白介素19(IL19)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Interleukin 19 (IL19)96TY17213Rattus norvegicus (Rat)
接觸蛋白1(CNTN1)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Contactin 1 (CNTN1)96TY17214Homo sapiens (Human)
接觸蛋白1(CNTN1)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Contactin 1 (CNTN1)96TY17215Mus musculus (Mouse)
多效生長(zhǎng)因子(PTN)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Pleiotrophin (PTN)96TY17216Homo sapiens (Human)
雙調(diào)蛋白(AREG)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Amphiregulin (AREG)96TY17217Mus musculus (Mouse)
雙調(diào)蛋白(AREG)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Amphiregulin (AREG)96TY17218Rattus norvegicus (Rat)
雙調(diào)蛋白(AREG)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Amphiregulin (AREG)96TY17219Oryctolagus cuniculus (Rabbit)
雙調(diào)蛋白(AREG)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Amphiregulin (AREG)96TY17220Bos taurus; Bovine (Cattle)
雙調(diào)蛋白(AREG)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Amphiregulin (AREG)96TY17221Equus caballus; Equine (Horse)
雙調(diào)蛋白(AREG)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Amphiregulin (AREG)96TY17222Chicken (Gallus)
雙調(diào)蛋白(AREG)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Amphiregulin (AREG)96TY17223Sus scrofa; Porcine (Pig)
Atonal同源物1(ATOH1)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Atonal Homolog 1 (ATOH1)96TY17224Homo sapiens (Human)
Atonal同源物1(ATOH1)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Atonal Homolog 1 (ATOH1)96TY17225Mus musculus (Mouse)
Atonal同源物1(ATOH1)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Atonal Homolog 1 (ATOH1)96TY17226Rattus norvegicus (Rat)
骨成型蛋白2(BMP2)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Bone Morphogenetic Protein 2 (BMP2)96TY17227Oryctolagus cuniculus (Rabbit)
結(jié)締組織生長(zhǎng)因子(CTGF)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Connective Tissue Growth Factor (CTGF)96TY17228Homo sapiens (Human)
結(jié)締組織生長(zhǎng)因子(CTGF)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Connective Tissue Growth Factor (CTGF)96TY17229Mus musculus (Mouse)
結(jié)締組織生長(zhǎng)因子(CTGF)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Connective Tissue Growth Factor (CTGF)96TY17230Rattus norvegicus (Rat)
結(jié)締組織生長(zhǎng)因子(CTGF)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Connective Tissue Growth Factor (CTGF)96TY17231Oryctolagus cuniculus (Rabbit)
結(jié)締組織生長(zhǎng)因子(CTGF)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Connective Tissue Growth Factor (CTGF)96TY17232Bos taurus; Bovine (Cattle)
結(jié)締組織生長(zhǎng)因子(CTGF)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Connective Tissue Growth Factor (CTGF)96TY17233Capra hircus; Caprine (Goat)
結(jié)締組織生長(zhǎng)因子(CTGF)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Connective Tissue Growth Factor (CTGF)96TY17234Chicken (Gallus)
結(jié)締組織生長(zhǎng)因子(CTGF)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Connective Tissue Growth Factor (CTGF)96TY17235Sus scrofa; Porcine (Pig)
骨成型蛋白2(BMP2)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Bone Morphogenetic Protein 2 (BMP2)96TY17236Homo sapiens (Human)
骨成型蛋白2(BMP2)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Bone Morphogenetic Protein 2 (BMP2)96TY17237Mus musculus (Mouse)
骨成型蛋白2(BMP2)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Bone Morphogenetic Protein 2 (BMP2)96TY17238Sus scrofa; Porcine (Pig)
骨成型蛋白2(BMP2)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Bone Morphogenetic Protein 2 (BMP2)96TY17239Rattus norvegicus (Rat)
骨成型蛋白2(BMP2)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Bone Morphogenetic Protein 2 (BMP2)96TY17240Capra hircus; Caprine (Goat)
骨成型蛋白2(BMP2)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Bone Morphogenetic Protein 2 (BMP2)96TY17241Chicken (Gallus)
轉(zhuǎn)化生長(zhǎng)因子β誘導(dǎo)蛋白(TGFβI)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Transforming Growth Factor Beta Induced Protein (TGFbI)96TY17242Bos taurus; Bovine (Cattle)
轉(zhuǎn)化生長(zhǎng)因子β誘導(dǎo)蛋白(TGFβI)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Transforming Growth Factor Beta Induced Protein (TGFbI)96TY17243Danio rerio (Zebrafish)
轉(zhuǎn)化生長(zhǎng)因子β誘導(dǎo)蛋白(TGFβI)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Transforming Growth Factor Beta Induced Protein (TGFbI)96T Y17244Homo sapiens (Human)
轉(zhuǎn)化生長(zhǎng)因子β誘導(dǎo)蛋白(TGFβI)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Transforming Growth Factor Beta Induced Protein (TGFbI)96TY17245Mus musculus (Mouse)
各種類型ELISA測(cè)定時(shí), 一般需經(jīng)過以下步驟:固相包被→加待測(cè)樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。